Genomic comparison of neodiprion sertifer nucleopolyhedrovirus nesenpv and neodiprion lecontei nucleopolyhedrovirus nelenpv showed that the hymenopteran baculoviruses had features in common and were distinct from other, fully sequenced lepidopteran and dipteran baculoviruses. Attacks of neodiprion sertifer on pinus contorta, eppo. Effect of mating disruption on reproductive behavior in. All structured data from the file and property namespaces is available under the creative commons cc0 license. Two naturally occurring nuclear polyhedrosis virus. In two reforestation areas with low populations of neodiprion sertifer, frost damage to new shoots on defoliated branches reached 97 % and 100 % respectively, while less than 10 % of the shoots on intact branches were damaged. Field evaluation of chiral isomers of the sex pheromone of. An lc50 was calculated at 1758 pibml also, the incubation time of the virus was dependent on its concentration. The difference in frost hardiness can be explained by reduced levels of. Diprionidae cocoons were planted singly 50 cocoons per site, in groups of 10 20 groups per site and in groups of. Capillary gc analysis showed that the natural pheromone. The genome of the neodiprion sertifer nucleopolyhedrovirus nesenpv, which infects the european pine sawfly, n. Stereochemically pure pheromone components of the pine sawfly, acetates of 2s,3s,7s and 2s,3r,7r3,7dimethylpentadecan2ol, were prepared by an improved synthetic procedure. Females of the pine sawfly neodiprion sertifer hymenoptera diprionidae usually avoid pinus pinea trees as host plants.
Linear regression analysis of probit mortality versus several concentrations of nucleo polyhedrosis virus of neodiprion sertifer resulted in the equation y 2. The alimentary canal of neodiprion abietis larvae is a straight tube divided into foregut, midgut, and hindgut. Neodiprion sertifer an overview sciencedirect topics. Progress of embryonic development in neodiprion sertifer geoffroy was examined in eggs collected. One of the parasites, the indigenous ichneumonid exenterus canadensis prov. Consequences of narrow temperature tolerance for a pinyon.
Pheromone related compounds in pupal and adult female pine. Presence of wolbachia in three hymenopteran species. The difference between doses was insignificant with. Alkaline protease assays of polyhedra revealed the presence of endogenous enzyme activity in the british isolate but not in the north american isolate. The larvae consume the needles, which causes decreased growth and. Putative secondary structure of rrnl gene in neodiprion sertifer. Bioassay of the nucleopolyhedrosis virus of neodiprion.
View the article pdf and any associated supplements and figures for a period of 48 hours. Persistence in soil and on foliage of nucleopolyhedrosis. A pair of muscular, chitinlined pouches branch off the anterior foregut and lie lateral to the alimentary canal. Temperature and crowding effects on virus manifestation. Metabolism and excretion of nitrogen during metamorphosis. Neodiprion sertifer, the european pine sawfly or red pine sawfly, is a sawfly species in the genus neodiprion. Pdf the effect of pyrethrum and bacillus thuringiensis. Diprionidae, is a widespread and economically important forest insect. The quantity of virus measured as the number of polyhedral inclusion bodies pibs produced and liberated at death from two diseased european pine sawfly neodiprion sertifer geoff.
Putative secondary structures of trna genes in neodiprion sertifer. Infestations of this insect have been controlled by weather, and frequently high percentages are destroyed by parasitic and predacinus insects, by. Diprionidae, were released and observed inside and outside control of areas treated with their sex pheromone for the purpose of mating disruption. Histology of the larval neodiprion abietis hymenoptera. Sodium dodecyl sulfate polyacrylamide gel electrophoresis of virus particle. Effectiveness of chemically decontaminated neodiprion. Neodiprion sertifer geoffroy larval populations were treated with high and low doses of a nucleopolyhedrosis virus npv product, neocheks. The release rates of the neodiprion sertifer geoffr. Comparisons of release rates, duration in the field, and catch efficiency of polyethylene and cotton roll dispensers for the sex pheromones of sawflies hymenoptera. Disruption of pheromone communication in the european pine. Neither the treatment nor the elimination of the contaminants reduced the effectiveness of the pib suspensions.
Diprionidae are two of the most important sawfly pests in italy, and both species are parasitized by the hymenopteran parasitoid dahlbominus fuscipennis zetterstedt. This page was last edited on 7 november 2015, at 15. Six plots of pine trees harboring high densities of n. Bacterial endosymbionts are currently studied for their high potential in strategies of biocontrol in a. Electroantennographic and single sensillum recordings were performed on male pine sawfly, neodiprion sertifer, antennae. Sawflies are a group insects related to wasps that get their common name from the sawlike appearance of the ovipositor, which females use to cut slits in stems or leaves to lay their eggs. The influence of pine volatile compounds on the olfactory. Media in category neodiprion sertifer the following 9 files are in this category, out of 9 total.
First field experiments with pheromone sticky traps for sawfly control in austria hymenoptera. The basic components and subsidiary factors comprising predation have been demonstrated in field and laboratory studies that analyzed the predation by small mammals of the european pine sawfly, neodiprion sertifer geoff. Diprionidae were studied by spraying egg clusters with a. It was found that 1 the outbreak was preceded by summer and autumn droughts, 2 it was. Geographic variation in the field response of male. The preference of sawfly females, measured as the proportion of. A summer frost caused light damage to pine shoots in some areas of central sweden in 1984. The european pine sawfly, neodiprion sertifer geoffr. Upon incubation of cocoons in darkness, most of the larvae from 4 to 15hour photoperiods metamorphosed without an intervening prepupal diapause, and emerged as adults 27 to 35 days after cocoon spinning. The percentage identity of trna gene sequences inuc% in. Pheromone mating disruption of the pine sawfly neodiprion. Metabolism and excretion of nitrogen during metamorphosis and egg production in the sawfly, neodiprion sertifer author links open overlay panel w. The use of a virus disease in the biological control of. Files are available under licenses specified on their description page.
Mixtures of the newly prepared compounds were tested in the field red pine forests in hyogo and nagano, japan for their attraction of neodiprion sertifer. Neodiprion sertifer, the european pine sawfly, has in the past attacked mainly pinus sylvestris. Download fulltext pdf download fulltext pdf download fulltext pdf the effect of pyrethrum and bacillus thuringiensis biopesticides on diprion pini l. Viral control of the european pine sawfly, neodiprion. Afforestation of areas in northern scotland with p. The maximum response was observed in mixtures of the ratio. Responses to the sex pheromone component 2s, 3s, 7s 3,7dimethyl2pentadecenyl diprionyl acetate sss.
Sawflies are important pests of various plant species. The 2s, 3s, and 7r isomers showed weak activity, but the other optical isomers were not attractive. Estimation of nuclear polyhedrosis virus produced in field. If you have any questions, or need the bot to ignore the links, or the page altogether, please visit this simple faq for additional information. The sex pheromone communication system of this species has been previously investigated in north america, japan and europe, with the acetate or propionate of the alcohol 2s,3s,7s3,7dimethyl2. This accidentally introduced pest is, as the name implies, native to europe and was first found in north america in 1925 in new jersey. Females of the european pine sawfly, neodiprion sertifer geoffr.
The european pine sawfly, neodiprion sertifer geoffroy hymenoptera. Sequence analysis of the genome of the neodiprion sertifer. Acidic precipitation increases egg survival in neodiprion. Pdf climate change and the risk of neodiprion sertifer. Neodiprion sertifer were studied in the field on 61 naturally regenerated scots pine trees. Canadian journal of zoology canadian science publishing. Oac, to the six other enantiomers of diprionyl acetate, and to the biosynthetic. The pine sawfly pheromone precursor 3,7dimethyl2pentadecanol diprionol was quantified by gas chromatography in different body parts of virgin female neodiprion sertifer. Neodiprion sertifer geoffroy has a wide temperature tolerance, experiencing egg mortality below. In contrast, this sawfly species is highly attracted by p. Pdf feeding behavior and performance of neodiprion.
External links modified february 2018 hello fellow wikipedians, i have just modified one external link on neodiprion sertifer. Among optical isomers of 3,7dimethylpentadecan2ol diprionol acetate or propionate tested as synthetic attractants, the 2s, 3s, and 7s isomers were most effective in attracting the males ofneodiprion sertifer in the field. Here, we investigated which pine volatiles might mediate this behavior by in situ sampling experiments and olfactometer laboratory tests. Larval population reduction due to neocheks was well over 90% in all sprayed plots 28 days after application, whereas overall protection of pinus resinosa ait. Polyhedral inclusion bodies pib were recovered in all plots from soil and foliage sampled at fixed time intervals within a 21month period from the initial date of spraying. Behaviour of male pine sawflies, neodiprion sertifer. As seen in figure 2, the viral concentrations, 5x109, 5x108 and 5x107 showed the same mortality, but the infection periods.
The european pine sawfly eps, neodiprion sertifer, is a common sight on certain pines in the spring in wisconsin. Posterior to the mouth, the foregut is further divided into the pharynx, esophagus crop, and proventriculus, all of which are lined with cuticle. Oac, to the behavioral inhibitor 2s, 3r, 7rdiprionyl acetate srr. Neodiprion sertifer geoffroy hymenoptera, diprionidae. Predation was restricted to the cocoon stage of the insect and only three species of small mammals were important predators sorex cinereus cinereus kerr, blarina. Pheromone mating disruption of the pine sawfly neodiprion sertifer. Males of the european pine sawfly, neodiprion sertifer geoffr.
Sequence analysis of the genome of the neodiprion sertifer nucleopolyhedrovirus. Genomic comparison of neodiprion sertifer and neodiprion. Effects of feeding by neodiprion sertifer fourcroy. Pdf sequence analysis of the genome of the neodiprion. The possibility of imperfect coincidence between the appropriate stages of neodiprion sertifer geoff. Unlimited viewing of the articlechapter pdf and any associated supplements and. The plantfeeding larvae often look like caterpillars or slugs, and many are quite noticeable because they often stay together to feed in groups and quickly cause noticeable defoliation on their hosts. Sex pheromone perception in male pine sawflies, neodiprion. Neodiprion sertifer geoffroy neodiprion swainei middleton. Half of these plots were resprayed in the second year of the study. Although native to europe, it was accidentally introduced to north america in 1925. The components of predation as revealed by a study of.
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